Basically what I am trying to achieve is overlay one stack with another, in a layer that maybe displayed or not. I know it is possible to overlay 2D images but is there a function to overlay stacks.
I have a stack in .raw format containing a 3D uCT scan and another stack in .raw format containing regions of interest. I want to overlay the ROIs onto the stack for all slices, but each slice is different. Is there a way to do this in ImageJ?
Thank you
You can use the command Image / Color / Merge channels. For example, load the two images, assign the uCT to the gray channel and the rois to the red channel.