Here's the code that I'm trying to run:
import pyautogui
r=pyautogui.locateOnScreen('C:\Users\David\Desktop\index.png',grayscale=False)
print r
It has to be a pixel-perfect match in order to be found. To allow for any sort of deviance you can invoke a confidence parameter.
For example:
loc = pyautogui.locateOnScreen(image, grayscale=True, confidence=.5)
However, in order to use the confidence parameter you have to have opencv_python installed. This is easy to install with pip:
./python -m pip install opencv_python
After that is in place, you should be able to account for minor differences.