It's very hard to find detailed but simple description of worker and I/O threads in .NET
What's clear to me regarding this topic (but may not be technically precise):
What is not clear:
The term 'worker thread' in .net/CLR typically just refers to any thread other than the Main thread that does some 'work' on behalf of the application that spawned the thread. 'Work' could really mean anything, including waiting for some I/O to complete. The ThreadPool keeps a cache of worker threads because threads are expensive to create.
The term 'I/O thread' in .net/CLR refers to the threads the ThreadPool reserves in order to dispatch NativeOverlapped callbacks from "overlapped" win32 calls (also known as "completion port I/O"). The CLR maintains its own I/O completion port, and can bind any handle to it (via the ThreadPool.BindHandle API). Example here: http://blogs.msdn.com/junfeng/archive/2008/12/01/threadpool-bindhandle.aspx. Many .net APIs use this mechanism internally to receive NativeOverlapped callbacks, though the typical .net developer won't ever use it directly.
There is really no technical difference between 'worker thread' and 'I/O thread' -- they are both just normal threads. But the CLR ThreadPool keeps separate pools of each simply to avoid a situation where high demand on worker threads exhausts all the threads available to dispatch native I/O callbacks, potentially leading to deadlock. (Imagine an application using all 250 worker threads, where each one is waiting for some I/O to complete).
The developer does need to take some care when handling an I/O callback in order to ensure that the I/O thread is returned to the ThreadPool -- that is, I/O callback code should do the minimum work required to service the callback and then return control of the thread to the CLR threadpool. If more work is required, that work should be scheduled on a worker thread. Otherwise, the application risks 'hijacking' the CLR's pool of reserved I/O completion threads for use as normal worker threads, leading to the deadlock situation described above.
Some good references for further reading: win32 I/O completion ports: http://msdn.microsoft.com/en-us/library/aa365198(VS.85).aspx managed threadpool: http://msdn.microsoft.com/en-us/library/0ka9477y.aspx example of BindHandle: http://blogs.msdn.com/junfeng/archive/2008/12/01/threadpool-bindhandle.aspx